AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human Early Endosome Antigen 1 (EEA1) IgG fraction polyclonal antibody

Catalog NumberPN-7090a-9
Product IdPN-7090a-9
Product NameRabbit anti-Human Early Endosome Antigen 1 (EEA1) IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Neurobiology)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (TPSSKKPVRVCDACFNDLQG) corresponding to amino acids 1392-1411 of human EEA1. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody can be used in ELISA (1: 50 dilution), immunocytochemistry (1: 100 dilution) and it recognizes EEA1 (162 kDa) in Western blot of HeLa lysate (1: 500 dilution). Early endosome antigen 1 (EEA1) is a peripheral membrane protein that contains a calmodulin-binding IQ motif and cysteine rich finger motif necessary for its specific localization to the early endosomes. These early endosomes are cytoplasmatic compartments which fuse the endocytic vesicles for redistribution of extracellular compounds to alternate destinations. EEA1 mediates the regulatory effects of 3'-phosphoinositides on membrane trafficking through its FYVE-type zinc finger domain.
FormulationSolution at 0.5 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesMu, F.T. et al. (1995) J. Biol. Chem. 270, 13503-13511. Stenmark, H. et al. (1996) J. Biol. Chem. 271, 24048-24054. Gaullier, J.M. et al. (2000) J. Biol. Chem. 275, 24595-24600.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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