AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human NFkappaB p52. IgG fraction polyclonal antibody

Catalog NumberPCA-2111a-9
Product IdPCA-2111a-9
Product NameRabbit anti-Human NFkappaB p52. IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Apoptosis)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (CYNPGLDGIIEYDDFK) corresponding to amino acids 4-19 of human NFkappaB p52. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody can be used in ELISA (1: 100 dilution), immunocytochemistry (1: 100 dilution) and it recognizes NFkappaB protein (52 kDa) in Western blot of HeLa cell lysate (1: 500). Nuclear factor kappa light chain enhancer in B cells (NF?B) is a transcription regulator that consists of two subunits, 50 kDa DNA binding subunit (p50) and 65 kDa transactivation subunit (p65/Rel1A). p52 subunit has an isoform p50 and both proteins are derived from p105 precursor. NFkappaB p52 forms dimers with other members of the NFkappaB/Re1 and it remains in the cytosol in an inactive form bounded to its inhibitor IkappaB. Upon stimulation, phosphorylation of IkappaB releases NFkappaB complex which is traslocated into de nucleus and regulates the expression of genes that participate in immune, apoptotic, and oncogenic processes.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesBours, V. et al. (1992) Mol. Cell. Biol. 12, 685-695. Heusch, M. et al. (1999) Oncogene 18, 6201-6208. Bours, V. et al. (2000) Biochem. Pharmacol. 60, 1085-1089. Xiao, G. et al. (2001) Mol. Cell. 7, 401-409.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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