| Catalog Number | PCA-2100a-9 |
| Product Id | PCA-2100a-9 |
| Product Name | Rabbit anti-Human C-erbB-2 IgG fraction polyclonal antibody |
| Temperature | -20°C |
| Unit | 1mg |
| Price | 425 |
| Availability | Available |
| Category | Growth Factors, Cytokines, Receptors and their Antibodies |
| Subcategory | Polyclonal Antibodies (Cancer/Apoptosis) |
| Description | IgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (AENPEYLGLDVPV) corresponding to amino acids 1243-1255 of human c-erbB-2/HER-2/neu oncoprotein. The IgG fraction was purified using Protein G-Sepharose. |
| Isotype | N/A |
| Mol Weight | N/A |
| Purity | N/A |
| Storage | Store at -20ºC. |
| Stability | Stable at least one year at -20ºC. Avoid repeated freezing and thawing. |
| Biological Activity | This polyclonal antibody can be used in ELISA (1: 100 dilution), immunocytochemistry (1: 100 dilution) and it recognizes c-erbB-2 protein (185 kDa) in Western Blot of HeLa cell lysate (1: 500 dilution). C-erbB-2 oncoprotein is a 185 kDa type I membrane tyrosine kinase receptor and belongs to the EGF receptor family. It plays an important role in the control of intracellular signaling events which are crucial for cell growth, differentiation and survival. The overexpression of the erbB-2 proto-oncogene is associated with an aggressive human adenocarcinomas, mainly breast and ovarian tumours. |
| Formulation | Solution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial. |
| References | Slamon, D.J. et al. (1989) Science 244 (4905), 707-712. Klapper, L.N. et al. (2000) Adv. Cancer Res. 77, 25-79. |
