AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human PUMA alpha (epitope a) IgG fraction polyclonal antibody

Catalog NumberPCA-2080a-9
Product IdPCA-2080a-9
Product NameRabbit anti-Human PUMA alpha (epitope a) IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Apoptosis)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (ARARQEGSSPEPVEG) corresponding to amino acids 2 - 16 of human PUMA isoform alpha. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody reacts in ELISA using the immunizing peptide and it recognizes PUMA alpha (23 kDa) and additional protein band of 60 kDa in Western Blot of HeLa cell lysate (1: 1,000 dilution). PUMA (p53 up-regulated modulator of apoptosis) belongs to the Bcl-2 family and may be a direct mediator of p53-induced apoptosis. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta, both bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesYu, J. et al. (2001) Mol. Cell 7, 763-682. Nakano, K. & Vousden, K.H. (2001) Mol. Cell 7, 683-694. Schuler, M. & Green, D.R. (2001) Bioch. Soc. Trans. 29, 684-688. Chipuk, J.E. & Green, D.R. (2006) 13, 994-1002.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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