AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human Cullin5 (CUL 5) IgG fraction polyclonal antibody

Catalog NumberPCA-2020a-9
Product IdPCA-2020a-9
Product NameRabbit anti-Human Cullin5 (CUL 5) IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Apoptosis)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (SNLLKNKGSLQFEDK) corresponding to amino acids 4-19 of human Cullin 5. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody can be used in ELISA (1: 500 dilution), immunocitochemistry (1:100 dilution) and it recognizes Cullin 5 (91 kDa) in Western blot of HeLa lysate (1: 1,000 dilution). Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 5 (CUL 5) is a component of E3 ubiquitin ligase complexes and it is involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. It may also form a cell surface vasopresin receptor and it may play a role in brast tumorigenesis.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesByrd, P.J. et al. (1997) Genome Res. 7, 71-75. Fay, M.J. et al. (2003) Mol. Cancer 2, 40,
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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