AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human Cullin4a (CUL 4a) IgG fraction polyclonal antibody

Catalog NumberPCA-2015a-9
Product IdPCA-2015a-9
Product NameRabbit anti-Human Cullin4a (CUL 4a) IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Apoptosis)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (MADEAPRKGSFSALVGRTNG) corresponding to amino acids 1-20 of human Cullin 4a. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody can be used in ELISA (1: 500 dilution), immunocitochemistry (1:100 dilution) and it recognizes Cullin 4A (75 kDa) in Western blot of HeLa lysate (1: 1,000 dilution). Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 4 (CUL 4) serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits of SCF. It plays a role in the polyubiquitination of CDT1 in response to radiation-induced DNA damage and may be important for subsequent DNA repair.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesChen, L-C. et al. (1998) Cancer Res. 58, 3677-3683. Dai, Q. & Wang, H. (2006) Cell Div. 1, 14. Higa, L.A et al. (2006) Cell Cycle 5, 1675-1680. Angers, S. et al. (2006) Nature 443, 590-593.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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