AUSTRAL Biologicals Catalog

Product Sheet: Rabbit anti-Human Cullin3 (CUL 3) IgG fraction polyclonal antibody

Catalog NumberPCA-2010a-9
Product IdPCA-2010a-9
Product NameRabbit anti-Human Cullin3 (CUL 3) IgG fraction polyclonal antibody
CategoryGrowth Factors, Cytokines, Receptors and their Antibodies
SubcategoryPolyclonal Antibodies (Cancer/Apoptosis)
DescriptionIgG fraction from rabbit polyclonal antibody obtained by immunizing rabbits with a synthetic peptide (MSNLSKGTGSRK) corresponding to amino acids 1-12 of human Cullin 3. The IgG fraction was purified using Protein G-Sepharose.
Mol WeightN/A
StorageStore at -20ºC.
StabilityStable at least one year at -20ºC. Avoid repeated freezing and thawing.
Biological ActivityThis polyclonal antibody reacts in ELISA (1: 100 dilution) and Western blot (1: 1,000) using the immunizing peptide. Cullins (1, 2, 3, 4a, 5 and 7) are a family of proteins that assemble a large number of ubiquitin ligases to catalyse polyubiquitiation, as well as binding to various specific factors to recruit substrates. Cullin 3 (CUL 3) is involved in the ubiquitination of cyclin E forming a tight complex with this protein which leads to its degradation by proteosomes.
FormulationSolution at 1 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.
ReferencesDu, M. et al. (1998) J. Biol. Chem. 273, 24289-24292. Singer, J.D. et al. (1999) Genes & Dev. 13, 2375-2387. Van den Heuvel, S. (2004) Curr. Biol. 14, R59-61. Sumara, I. et al. (2007) Dev. Cell. 12, 887-900.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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