AUSTRAL Biologicals Catalog

Product Sheet: Anti-human Mediator 10 (MED10 or HNUT2) IgG fraction (monoclonal)

Catalog NumberTM-901Z-55
Product IdTM-901Z-55
Product NameAnti-human Mediator 10 (MED10 or HNUT2) IgG fraction (monoclonal)
CategoryProteins and Antibodies for Transcription Research
SubcategoryProteins and Antibodies
DescriptionIgG fraction from mouse monoclonal antibody (clone 8A1/F4) obtained by immunization of mice with human MED10 (25 kDa). The IgG fraction was purified using Protein G-Sepharose.
StorageStore at -20°C.
StabilityStable for at least one year. Avoid repeated freezing and thawing
Biological ActivityThis antibody can be used in ELISA assays (1: 8,000 dilution) and Western blot. It recognizes human MED10 (hNut2), a subunit protein of Mediator (MED) complex. The Mediator is a multiprotein coactivator required for activation of RNA polymerase II transcription by DNA binding transcription factors. The MED10 has been assigned to the middle domain of Mediator and it interacts physically with the carboxy-terminal domain (CTD) of largest RNA polymerase II subunit according to electron microscopic data.
FormulationSolution at 1.0 mg/mL in PBS
ReferencesGuglielmi, B. al (2004) Nucleic Acids Res. 32, 5379 – 5391; Bourbon, H-M. et al (2004) Mol Cell 14, 553 – 557; Lewis, B. & Reinberg, D. (2003) J. Cell Sci. 116, 3667 – 3675; Boube, M. et al (2002) Cell 110, 143 – 151; Woychik, N. & Hampsey, M. (2002) Cell 108, 453 – 463.
All products are for research use ONLY in laboratory animals and in vitro testing. NOT for diagnostic or human use.

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